GCGR/CRE-Luc/HEK293
CBP71345
詢(xún) 價(jià)
索取protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫(kù)
| I. Background | |
|
胰高血糖素是一種胰腺肽激素，作為胰島素的反調(diào)節(jié)激素，刺激葡萄糖通過(guò)肝臟釋放， 維持葡萄糖穩(wěn)態(tài)。GCGR 是一種 B 類(lèi) GPCR，最初被描述為與腺苷酸環(huán)化酶功能相連的胰 高血糖素結(jié)合體。它在維持葡萄糖穩(wěn)態(tài)中起關(guān)鍵作用，因此被認(rèn)為是治療糖尿病的一個(gè)有 價(jià)值的靶點(diǎn)。 |
|
| II. Description | |
|
GCGR CRE-Luc HEK293 報(bào)告基因藥靶模型很好的模擬了體內(nèi) GCGR 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程， 原理見(jiàn)下圖所示。
|
|
| III. Introduction | |
| Expressed gene: |
GCGR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GCGR |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: |
Embryonic kidney
|
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
V. Representative Data Figure 2. Recombinant GCGR/CRE-Luc/HEK293 stably expressing GCGR.  |
|
Figure 4. HTRF cAMP Assay with GCGR CRE-Luc HEK293( C21).
